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Staine And Types Of Stains Pdf

staine and types of stains pdf

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Special Stains – Which One, How and Why? Part II: Connective Tissue

The following points highlight the top five types of Staining. The types are: 1. Simple Staining 2. Differential Staining 3. Gram Staining 4. Acid Fast Staining 5. Endospore Staining.

In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. We have already alluded to certain techniques involving stains and fluorescent dyes, and in this section we will discuss specific techniques for sample preparation in greater detail. Indeed, numerous methods have been developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope. Here, we will focus on the most clinically relevant techniques. In clinical settings, light microscopes are the most commonly used microscopes.

Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. If you continue browsing the site, you agree to the use of cookies on this website. See our User Agreement and Privacy Policy. See our Privacy Policy and User Agreement for details. Published on Feb 3, Staining Techniques used in Microbiology.

3.4: Summary of Common Bacterial Staining Techniques

Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology the study of tissue under the microscope and in the medical fields of histopathology , hematology , and cytopathology that focus on the study and diagnoses of disease at a microscopic level. Stains may be used to define biological tissues highlighting, for example, muscle fibers or connective tissue , cell populations classifying different blood cells , or organelles within individual cells. In biochemistry it involves adding a class-specific DNA , proteins , lipids , carbohydrates dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry , and to flag proteins or nucleic acids in gel electrophoresis. Staining is not limited to biological materials, it can also be used to study the structure of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.

If you have viewed this educational webinar, training or tutorial on Knowledge Pathway and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. Connective tissue supports the body by providing a matrix that connects and binds the cells and organs. There are three types of connective tissue in the body. Collagen is a strong protein and is a main component of ligaments and tendon. Elastic fibers are located in the skin and walls of blood vessels.

The microscope is a very important tool in microbiology, but there are limitations when it comes to using one to observe cells in general and bacterial cells in particular. Two of the most important concerns are resolution and contrast. Contrast, however, can be improved by either using a different type of optical system, such as phase contrast or a differential interference contrast microscope, or by staining the cells or the background with a chromogenic dye that not only adds contrast, but gives them a color as well. There are many different stains and staining procedures used in microbiology. Some involve a single stain and just a few steps, while others use multiple stains and a more complicated procedure. Before you can begin the staining procedure, the cells have to be mounted smeared and fixed onto a glass slide. A bacterial smear is simply that—a small amount of culture spread in a very thin film on the surface of the slide.


Types of staining techniques. Simple staining. Differential staining. (Use of of single stain) (Use of two contrasting stains) Direct. Indirect. Separation. Visualization. (Positive) (Negative) into groups. of structures. Gram stain. Flagella stain. Acid fast. Capsule stain.


Top 5 Types of Staining (With Diagram) | Microbiology

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Differential Staining Techniques

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4 Comments

  1. Gifford H.

    27.11.2020 at 04:29
    Reply

    Show Credits Hide This Giemsa stained micrograph depicts an example of a slightly acidic slide that yielded a pink colored resultant stain.

  2. Monique O.

    02.12.2020 at 15:59
    Reply

    Uses an acidic stain: Congo red or Nigrosin and a basic stain: crystal violet or safranin.

  3. Soren B.

    03.12.2020 at 03:45
    Reply

    The presence of acid fast bacilli in multiple specimens was investigated comparatively with Ziehl-Neelsen ZN and fluorescence microscopy FM staining in order to determine sensitivity in detecting tuberculosis TB.

  4. Joe P.

    05.12.2020 at 11:22
    Reply

    Show Caption Hide Anthrax gram stain.

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